The results showed that compared with those of the control (n = 10), GSDMD-FL (P < 0.01) and GSDMD-N (P < 0.05) levels were significantly increased in the thyroid tissues of HT patients (n = 20; Figures 1A,B), as indicated by immunoblot analysis, and the level of GSDMD expression in the thyroid tissues of HT patients (n = 20) was also higher than that in controls (n = 5; P < 0.001; Figures 1C,D), as indicated by IHC analysis. This evidence concerns the gene GSDMD and hematocrit.