Although direct regulation of CHKα by TGFβ-signaling has not been reported to date, the expression of EMT transcription factors, including ZEB1, TWIST1 and SNAI1, and the EMT target genes vimentin and N-cadherin were found to be associated with dysfunctional choline metabolism in glioblastoma cells. This evidence concerns the gene TGFB1 and glioblastoma.