Moreover, immunofluorescence analysis of mouse tumour tissues demonstrated that the number of cells with MELK and Ki‐67 positive staining was lower in the OTSSP167 injection group than in the control group, while the number of cells with p‐ATM and p53 positive staining was higher (Figure 7F‐I), which was consistent with the in vitro and in vivo MELK inhibition results. This evidence concerns the gene TP53 and neoplasm.