Genes that could best classify TB patients and TST- were IL7R, PRF1, NLRP1, CD3E, CCR7, FCGR1A, IL5, TLR9, BLR1, CD19, NLRP12, NOD2, PTPRCv1, GNLY, TLR5, NCAM1 and RAB33A with AUCs of 0.97, 0.94, 0.94, 0.93, 0.93, 0.93, 0.91, 0.89, 0.88, 0.87, 0.87, 0.83, 0.83, 0.81, 0.80, 0.80 and 0.80 respectively (Fig 2B). The gene discussed is PRF1; the disease is tuberculosis.