CPSF4 and hepatocellular carcinoma: Based on both assays, semi-recombinant holo U7 snRNP (Figure 3A, lane 1) contained all recombinant subunits of the Sm ring, Δ51 FLASH, endogenous HCC (symplekin, CPSF100, CPSF73, CstF64) and the remaining CPSF subunits (CPSF160, WDR33, Fip1, CPSF30).