Based on both assays, semi-recombinant holo U7 snRNP (Figure 3A, lane 1) contained all recombinant subunits of the Sm ring, Δ51 FLASH, endogenous HCC (symplekin, CPSF100, CPSF73, CstF64) and the remaining CPSF subunits (CPSF160, WDR33, Fip1, CPSF30). The gene discussed is CPSF2; the disease is hepatocellular carcinoma.