To evaluate the effect of ASCT2 on HNSCC glutamine uptake, we performed lentiviral transduction with a control shRNA or one of two different shRNAs (shASCT2-1 and shASCT2-2), and protein knockdown was verified in SCC15 and FaDu HNSCC cells by western blotting (Fig. 1c, d). The gene discussed is SLC1A5; the disease is head and neck squamous cell carcinoma.