Therefore, we assessed the expression level of EMT-inducing transcription factors in shNaV1.5 compared to more invasive shCTL breast cancer cells, and identified that SNAI1 expression was specifically and significantly reduced by 69.4% (p < 0.001), while the expression of other EMT-promoting transcription factors ZEB1, ETS1 and TWIST1 was not affected (Fig. 1e). The gene discussed is TWIST1; the disease is breast cancer.