It is noteworthy that ASCL1 protein expression was higher in the MYCN-amplified neuroblastoma cell lines, Kelly and CHP-134, than in the nonamplified cell lines, SK-N-SH and its subclone SH-SY5Y (Fig. 3b), which overexpress MYC (c-MYC)30. This evidence concerns the gene MYCN and neuroblastoma.