In HepG2 and SMMC-7721 xenograft models, immunohistochemistry and western blot analysis of the tumor samples showed that in accordance with in vitro results, sanguinarine could inhibit the expression change of HIF-1α (Fig. 8a), EMT markers, including E-cadherin and Snail in HepG2 (Fig. 8b) so as to N-cadherin, Vimentin, and Snail in SMMC-7721 xenograft models (Fig. 8c). This evidence concerns the gene CDH2 and neoplasm.