PINK1 and hepatocellular carcinoma: To confirm that PINK1 is playing a role in mitochondrial biogenesis in HCC cells, we established in Huh7-NTC and -shPINK1 cells (Supplementary Fig. S4) and performed Nonyl Acridine Orange (NAO) staining to evaluate the mitochondrial mass in 20 and 1% O2 conditions.