SYK and breast carcinoma: Immunofluorescence analysis of MCF7 (Syk+/E-Cdh+) and MDA-MB-231 (Syk−/E-Cdh−) breast cancer cells transfected with WT or Mut E-Cdh-GFP showed that WT E-Cdh was mainly localized at AJ, whereas Mut E-Cdh-GFP was predominantly expressed in intracellular vesicles (Figure 3b).