To investigate whether ALDH3A1 induces stemness and influences the expression of molecules involved in tumor immunity, we employed the following tumor cell lines: a) WM266-4, metastatic melanoma cells BRAF wild type, b) HCC4006, metastatic NSCLC cells, c) MEL4478D, cells from a patient with melanoma BRAF wild type (gently provided by Prof. Michele Maio, University of Siena). The gene discussed is ALDH3A1; the disease is melanoma.