ITIH4 and HIV-1 infection: Since both the cellular and humoral arms of the immune system are thought to be necessary to control HIV-1 infection [53], the humoral responses elicited after immunization with DNA-gp120/MVA-B and DNA-gp120/MVA-LEO160-gp120 were also analyzed, quantifying by ELISA the total IgG levels of antibodies against HIV-1 gp120 protein (clade B, isolate BX08) in pooled sera obtained from mice 10 days post-boost (Figure 7).