To examine whether EV71 infection was sufficient to induce IFNβ expression in neural cells, human glioblastoma cell line (SF268) and neuroblastoma cell lines (IMR32 and SH-SY5Y) were cultured and infected with EV71 at a multiplicity of infection (MOI) of 40, and the infected cells were harvested at different time points. This evidence concerns the gene IFNB1 and infection.