Consistent with literature data, IHC performed on HCC specimens showed HIF-2α immune-positivity in the same areas also positive for NAE1 and SB3 (Figure 4G), with statistical analyses revealing that the highest levels of NAE1 mRNA were detected in the subclass of HCC patients with highest (> median value) levels of HIF-2α transcripts (Figure 4H). Here, NAE1 is linked to hepatocellular carcinoma.