Finally, analyzing HCC nodules from rodent model of hepatocarcinogenesis based on the administration to mice of the hepato-carcinogen dimethyl-nitrosoamine (DEN) in combination with the CDAA diet, we observed that (i) HIF-2α transcript levels were up-regulated in HCC nodules (Figure 5A); (ii) there was a positive correlation (Spearman r coefficient = 0.5238, p < 0.01) between HIF-2α and SB3 (Figure 5B); (iii) the highest levels of NAE-1 transcripts were found in the sub-class of nodules carrying higher (> than median value) HIF-2α expression (Figure 5C). This evidence concerns the gene NAE1 and hepatocellular carcinoma.