Additionally, the autophagy inhibitor 3-MA and lysosome inhibitor CQ further aggravated CCH-induced increased protein levels of NLRP3, cleaved caspase-1 and cleaved IL-1β, indicating autophagy might participate in CCH-induced activation of the NLRP3 inflammasome and subsequent release of IL-1β. The gene discussed is CASP1; the disease is columnar cell hyperplasia of the breast.