Through a lot of clinical trials with DCs via various routes (intradermal, intranodal, intravenous, subcutaneous, intratumoral) [212], cancer immunotherapy efficacy by DC vaccine was limited mainly due to inhibition of immune response by tumor-secreted TGF-β and FOXP3 related Treg cells and low quality of DC production [213]. This evidence concerns the gene FOXP3 and cancer.