To analyze TLR activation in monocytes from T2D patients and healthy subjects, further experiments were conducted to evaluate the IL-1β, IL-6, IL-8, IL-10, and TNF-α levels induced by TLR ligands, Pam3Cys or LPS, or M. tuberculosis infection in monocytes from T2D patients with an HbA1c of 9% and above (poor glycemic control) and healthy subjects. The gene discussed is TNF; the disease is type 2 diabetes mellitus.