In accordance with these results, the number of GFP-RFP-LC3-II-positive puncta in PCDH17-transfected CRC cells was significantly higher than that in the control cells after 24 h exposure to 20 μM 5-FU, whereas little signal was observed after treatment with the autophagy inhibitor 3-MA (3-methyladenine) (Fig. 3b, c). The gene discussed is MAP1LC3A; the disease is colorectal carcinoma.