For example, CD8+ T cells are involved in ZIKV clearance.36,37 CD4+ T cells, in particular their IFN-γ-producing activities, are known to promote humoral immune responses, facilitate virus control, and protect host from ZIKV infection.38–40 In this study, we demonstrated that, in both WT B6 and AB6 mice, the ZIKV NS4B-C100S mutant induced more robust Th1-type cellular immune responses than either the parent WT ZIKV or ZIKV-3′UTR-Δ10 mutant. This evidence concerns the gene CD4 and Zika virus infectious disease.