Xiang-Shan Fan in an IHC study of 132 samples (comprising 12 normal colon mucosa, 18 adenomas and 102 colorectal cancer cases) showed a significantly higher expression of Lgr5 in cancerous tissues but similar to our observation, this expression was not associated with pathological characteristics like differentiation grade, TNM status, invasion or age of participants but a higher expression was determined in female subjects (25). This evidence concerns the gene LGR5 and adenoma.