S1P has been shown to be involved in the vascular tube formation process, with roles in regulating vascular maturation 41 and permeability.42 In this experiment, we found that exogenous S1P increased eNOS and iNOS in the Ad‐SphK1 group and increased the levels of NO in brain tissue (Figure 4), which indicated that SphK1/S1P may influence angiogenesis and neuroinflammation in the context of cerebral ischemia‐reperfusion injury. This evidence concerns the gene NOS2 and Cerebral ischemia.