In C57BL/6J mice, RNAscope in situ hybridization with probes specific for neurons (Syp), astrocytes (Slc1a3) and EphA4 (Epha4) showed that at 1 week post-stroke, Epha4 was expressed in 61% of Syp + neurons (Fig. 2A and B) and 15% of Slc1a3+ astrocytes (Fig 2D and E). This evidence concerns the gene EPHA4 and stroke disorder.