Various amounts of WT and mutant RNF43 plasmids were also co-transfected at 1:1 ratio into two colon cancer cell lines (DLD1 and LoVo) as well as into STF-RZ-DKO cells, and WB analysis showed that the two proteins were detected at nearly identical levels across the range of expression (Fig. 2f). Here, RNF43 is linked to malignant colon neoplasm.