RNF43 and colonic neoplasm: Various amounts of WT and mutant RNF43 plasmids were also co-transfected at 1:1 ratio into two colon cancer cell lines (DLD1 and LoVo) as well as into STF-RZ-DKO cells, and WB analysis showed that the two proteins were detected at nearly identical levels across the range of expression (Fig. 2f).