This showed that overexpression of EDEM1 selectively stabilized a del52 CALR/CANX complex, as seen in Figure 6D, whereas the overexpression of EDEM3 did not significantly impact on the formation of mutant CALR/CANX complexes, but increased the WT CALR/CANX complex, as seen in Figure 6E. These results show that the expression of CALR variants is dependent on a proximal ER quality control system, most likely including EDEM3, TXNDC11, and DNAJC10, which, when deregulated in MPN, might lead to sufficient stabilization of the proteins to yield constitutive MPL activation. Here, EDEM3 is linked to myeloproliferative neoplasm.