KRAS and colorectal carcinoma: However, in our experiments, both parental lines, HCT116 and DLD-1, harbored a heterozygous point substitution Gly13Asp in the KRAS proto-oncogene, which is mutated in up to 50% cases of CRC [61], while Amankwatia et al. explored the miR-224-5p effect in a HCT116 wild-type KRAS background.