ACE2 and hydrops fetalis: In line with previous studies showing that high-fat intake activates the RAS [22,44], our results demonstrated that HF diet increased renal mRNA expression of Agt and Ace. Currently, there are two counterbalancing axes of the RAS: the classical ACE–Ang II–AT1R axis that promotes vasoconstriction and the non-classical ACE2–angiotensin (1–7)–MAS axis responsible for vasodilatation [13].