To understand the mechanism by which the antigen-pulsed DC vaccines are superior to the antigen-adjuvant vaccines, we further determined their effects on the immunogenicity, anti-tumor factors, and cytokine levels in the serum, the proportions of activated splenic CD8+ T cells and CD44+ CD62L+ memory T cells, and the proportions of infiltrating T cells and inhibitory T cells in the tumor microenvironment in mouse LL2 xenograft models. This evidence concerns the gene CD8A and neoplasm.