To determine whether the S-nitrosylation of HDAC2 mediates IFNα-dependent transcriptional activation of ISGs in tumor cells, Over-NOS1 (A375) cells were transfected with HDAC2-WT, HDAC2-C262A/C274A plasmids and stimulated with IFNα for 6 h. The gene discussed is IFNA1; the disease is neoplasm.