We used Rat 4x44K gene expression array to detect gene profiles of liver tissues from the male rats following 14 weeks of high-fat diet, we characterized the metabolic perturbations and metabolic pathway profiles alterations induced by NAFLD in the rat and found biological metabolism including regulation of gluconeogenesis, lipid biosynthetic process were over-represented and genes associated with pancreatic secretion pathway, adipokine metabolic pathway and insulin signaling pathway were down-regulated compared to NC group. Here, INS is linked to metabolic dysfunction-associated steatotic liver disease.