In addition, after GO and Pathway analyses, Four genes (IGFBP7, Notch1, HMGCR and ACACB) which were involved in both glucose and lipid metabolism were screened out, and they were supposed to associate with the vicious cycle between glycolipid metabolism which may aggravate the progression of NAFLD. This evidence concerns the gene HMGCR and metabolic dysfunction-associated steatotic liver disease.