To examine the basic mechanism of how Calebin A blocks TNF-β-promoted malignancy of CRC cells, we evaluated whether the effects of Calebin A on CRC cells in TNF-β-induced pro-inflammatory tumor microenvironments was associated with the suppression of proinflammatory transcription factor NF-κB phosphorylation and NF-κB-regulated gene proteins linked with tumor metastasis. This evidence concerns the gene NFKB1 and colorectal carcinoma.