NFKB1 and colorectal carcinoma: Thus, we examined if a reduction in cysteine residues by DTT (dithiothreitol) in p65-NF-κB might influence the interaction of p65-NF-κB to the DNA binding in the presence or absence of Calebin A. Interestingly, we showed that the inhibition of Calebin A on p65-NF-κB interaction to DNA was prevented by DTT in all three distinct CRC cell lines (HCT116, RKO, SW480) (Figure 6).