This was consistent with the upregulation of the majority of genes in EGF-EGFR, and its downstream pathways (PI3K-Akt, Rho GTPase, RAP1, and regulation of actin cytoskeleton) in PC3 revealed by our single-cell RNA-seq (scRNA-seq) data on prostate cancer and its prohibition by tyrphostin (AG 1478), which resulted in suppression of migration and invasion (Horning et al., data not shown). This evidence concerns the gene AKT1 and prostate cancer.