SPP1 and ocular melanoma: As observed in OM treated cells (Fig 1C–1E), RT-PCR and immunoblotting analyses demonstrated an increase in the expression levels of osteoblast marker genes at mRNA (ALP, OPN, and OCN; Fig 2B) and protein (Col 1, OPN, RUNX2, Osterix; Fig 2D) levels as compared with cells grown in BM with no MSM ((-), lane 1 in Fig 2B and 2D).