As aproof-of-concept in a more complex biomimetic model, we demonstrated that selectiveinhibition of mechanical signaling through TRPV4 also suppressed pulmonary barrier leakagein a human Lung Alveolus Chip (Alveolus Chip) that has been previously shown to modelmultiple human pulmonary diseases with high fidelity, including pulmonary edema andpulmonary thrombosis.13,40,41. The gene discussed is TRPV4; the disease is pulmonary edema.