ANXA5 and neoplasm: After treatment with IC50 concentration of NV669 for 24 h, the percentage of cells at early (annexin-V positive cells, propidium iodide negative cells) and late (annexin-V positive cells, propidium iodide positive cells) stages of apoptosis significantly increased from control to NV669-treated cells, independently of the tumor cell origin (Figure 3A).