BRCA1 and neoplasm: More recently, we showed that BRCA1 c.594−2A > C occurs in cis with BRCA1 c.641A > G and should not be considered as a high-risk pathogenic variant because the out-of-frame splicing alteration did not affect the predominant alternative spliced event, Δ(E9_E10), which retains tumor suppressor activity (de la Hoya et al., 2016).