In this study, we also identified several key enzymes in glycolysis, gluconeogenesis, and phenylalanine metabolism, such as phosphoglycerate kinase 1 (PGK1), triosephosphate isomerase (TPI1) and aldehyde dehydrogenase family 1, member A3 (ALDH1A3), indicative of the regulation of cancer cell metabolism by S-nitrosylation during PDAC development. This evidence concerns the gene TPI1 and cancer.