The most common grade 3 or higher irAEs were lung infection (2.63%; 95% CI, 0.99–6.8%), amylase increased (1.69%; 95% CI, 0.54–5.09%) and hyperglycemia (0.99%; 95% CI, 0.41–2.36%) for NIVO and ALT increased (6.26%; 95% CI, 4.32–9.00%), colitis (5.21%; 95% CI, 3.24–8.26%) and AST increased (5.07%; 95% CI, 3.46–7.37%) for NIVO+IPI. This evidence concerns the gene GPT and colitis.