Importantly, stratification of the study group based on the TG/HDL-C index revealed association of higher levels of ANKRD26 methylation and reduced gene expression with high-cardio-metabolic risk, independent of BMI, indicating that alterations of both the DNA methylation and mRNA expression at the ANKRD26 gene correlate to increased risk of insulin resistance and cardiovascular disease in humans. Here, ANKRD26 is linked to cardiovascular disorder.