Furthermore, in osteosarcoma cells, TRPM8 knockdown induces Ca2+imbalance, inhibition of protein kinase B (Akt)-Glycogen synthase kinase (GSK)-3β, extracellular signal–regulated kinases (ERK)1/2 and FAK pathways, decreases proliferation, invasion, and migration, and improves apoptosis induced by epirubicin [62]. This evidence concerns the gene AKT1 and osteosarcoma.