As listed in Supplementary Table S5, multifocal disease (P=0.002), bilobar tumor location (P=0.001), largest nodule size >3.3 cm (P=0.003), portal vein invasion (P<0.001), higher ECOG performance status (P<0.001), higher BCLC stage (P<0.001), RBC abnormal (P=0.025), abnormal ALB (P=0.025), abnormal TBA (P=0.024), abnormal ALP (P=0.009), abnormal AFP (P=0.003), and abnormal CEA (P=0.004) were factors for predicting worse OS. The gene discussed is AFP; the disease is neoplasm.