FAM53A was expressed at significantly lower levels in the p53-wild-type breast cancer cell line MCF-7 compared with the normal human mammary epithelial cell line MCF-10A, whereas in the p53-mutant breast cancer cell lines T47D, MDA-MB-231, BT-549, and BT-474, FAM53A was highly expressed, particularly in MDA-MB-231 cells (Figure 1B). This evidence concerns the gene TP53 and breast carcinoma.