Furthermore, we found that six selected miRNAs in this study may also act as regulators of classical genes involved with RASopathies, such as hsa-miR-146a-3p (SPRED1 and RAF1), hsa-miR-196a-3p (RAF1, NF1, BRAF, and SPRED2), hsa-miR-548l (RASA1, RASA2, RRAS2, KRAS, MAP2K1, and SPRED1), hsa-miR-449b-5p (RRAS, SHOC2, and MAP2K3), hsa-miR-575 (SHOC2), and hsa-miR499a-3p (RASA1) as shown in Figure 2 and Supplemental Table 3. This evidence concerns the gene SPRED1 and RASopathy.