We also observed more HC-like cells after Dox-induced NOTCH activation, MHY1485 treatment together with ad-Atoh1 infection than by Dox-induced NOTCH activation and ad-Atoh1 infection in cultured rtTA/tet-NICD adult cochlea (Fig. 7l, m, o), suggesting that MHY1485 and NICD work synergistically to promote HC regeneration. This evidence concerns the gene ATOH1 and infection.