At different time points post‐infection, we found that EV71 VP1 expression in HEK293T‐shSAMHD1 cells and supernatants was higher than those in HEK293T‐pLKO.1 control cells and supernatants at 48 h post‐infection (Fig 1E), indicating that SAMHD1 inhibits EV71 replication. Here, SAMHD1 is linked to infection.