PGR and intrahepatic cholangiocarcinoma: In a breast cancer patient with HER2+/ER+/PR+ subtype, simultaneous detection of HER2/PR expression by triple IF (HER2/PR/Hoechst) and cytokeratin expression of CTCs by ICC could be confirmed (Fig. 3C), indicating the potential utility of this method for the identification of breast cancer subtypes in the clinical setting.