Similar to our dMRI findings, we observed significant changes in Thy1-YFP expression between the two cohorts along medial prefrontal tracts traversing the striatum (p = 0.00017) that would otherwise be difficult to detect with a region of interest (ROI)-based approach (Supplementary Fig. 13c, d, example tract profiles for stroke and control mice in Supplementary Fig. 13e). Here, THY1 is linked to Stroke.