PC and neoplasm: The activity of PC (pyruvate carboxylase), the enzyme responsible for the conversion of pyruvate into oxaloacetate, was elevated in NSCLC tumours [28,33], and its silencing significantly decreased the proliferative and colony-forming capacity of NSCLC cell lineages and reduced tumour growth in murine xenograft models, suggesting a dependence on PC-mediated and TCA cycle-based anaplerosis [33].