Upregulation of the metabolic genes (ALDH18A1, CAD, CHKA, POLD4, PSPH, and SQLE) and aberrant expression of cancer-related genes (ALCAM, ITGA6, DDIT3, MAP3K6, and PAK1) were found in mouse fed with high-fat-high-cholesterol similar to human NASH-HCCs [54]. This evidence concerns the gene PSPH and metabolic dysfunction-associated steatohepatitis.