Our method discovered the potential genes in HBx(p53-) and Src(p53-) diet-induced obesity, which might represent the transition stages from NASH to early HCC (Tables S6 and S7), as well as the genes for HCC from overfed HBx,Src(p53) fish (Table S8). Here, SRC is linked to obesity due to melanocortin 4 receptor deficiency.