Actually, the extensive diet treatment for 16 weeks for WT, HBx(p53-) and Src(p53-) promoted HCC formation; however, the HBx,Src(p53-) diet-induced obesity for 16 weeks reduced the HCC formation, which may due to the self-healing of zebrafish reported from various transgenic fish lines from our lab and others [6,32,33,48,49]. This evidence concerns the gene TP53 and hepatocellular carcinoma.