In total, 32 genes were identified as miR-99a-3p-controlled genes, and 10 genes (STAMBP, TIMP4, TMEM14C, CANX, SUV420H1, HSP90B1, PDIA3, MTHFD2, BCAT1, and SLC22A15) significantly predicted 5-year overall survival in patients with HNSCC. The gene discussed is HSP90B1; the disease is head and neck squamous cell carcinoma.